| Rank | Name | Time | Year |
| 1 | Erica Long | 25.10 | 1997 |
| 2 | Cami Kelsay | 26.10 | 2018 |
| 3 | Samantha Frazier | 26.38 | 2024 |
| 4 | Kloie Doublin | 26.39 | 2018 |
| 5 | Jennifer Stone | 26.40 | 1988 |
| 6 | Natalie McWhorter | 26.48 | 2003 |
| 7 | Anne Golden | 26.73 | 1984 |
| 8 | Erin Bullington | 26.75 | 2005 |
| 9 | Lauren Klem | 26.76 | 2024 |
| 10 | Ellie Stringer | 26.78 | 1997 |
| 11 | Addie Martin | 27.0 | 2024 |
| 12 | Olivia Nicoloff | 27.07 | 2022 |
| 13 | Jennifer Briggs | 27.10 | 1998 |
| 14 | Dolly VanCleave | 27.20 | 1982 |
| Christina Thompson | 27.20 | 1991 |
| 16 | Shannon Eades | 27.24 | 1988 |
| 17 | Ainsley Botkin | 27.29 | 2024 |
| 18 | Angie Kutruff | 27.30 | 1982 |
| 19 | Tori Hamner | 27.40 | 2009 |
| 20 | Brooke McClure | 27.43 | 1997 |
| 21 | Sara Doyle | 27.50 | 1982 |
| Christina Linville | 27.50 | 1982 |
| 23 | Chris Teike | 27.52 | 1987 |
| 24 | Sarah Craciunoiu | 27.54 | 1994 |
| 25 | Destinee Smith | 27.62 | 2009 |
| Rank | Name | Time | Year |
| 1 | Brian Murr | 21.74 | 2011 |
| 2 | Richard Jordan | 21.90 | 1989 |
| 3 | Brent Waymire | 22.1* | 1972 |
| Ron Dougherty | 22.1* | 1967 |
| 5 | Travis Murr | 22.40 | 2017 |
| Don Clarkston | 22.4* | 1966 |
| 7 | Bart Hunter | 22.50 | 1993 |
| Andy Dunn | 22.50 | 2007 |
| 9 | Keith Fechtman | 22.53 | 1997 |
| 10 | Alex Leugers | 22.59 | 2024 |
| 11 | Mark Findley | 22.70* | 1969 |
| 12 | Sean Packer | 22.79 | 1989 |
| 13 | Brian Murr | 22.90 | 2010 |
| 14 | Jake Moran | 22.95 | 2009 |
| 15 | Riley McCann | 23.05 | 2018 |
| 16 | Gary White | 23.10 | 1981 |
| 17 | Josh Cox | 23.14 | 2015 |
| 18 | Nick Hamner | 23.33 | 2003 |
| 19 | Sam Crenshaw | 23.40 | 1982 |
| Raven Montalvo | 23.40 | 2004 |
| 21 | Jason Wienhorst | 23.41 | 1998 |
| 22 | Brian Shepard | 23.50 | 1988 |
| Chris Wagner | 23.50 | 2002 |
| 24 | Scott Whittenbarger | 23.52 | 1998 |
| 25 | Derek Deboy | 23.59 | 2000 |
| | | |
| *=Converted times | |