| Rank | Name | Time | Year |
| 1 | Aubrey Runyon | 14.52 | 2024 |
| 2 | Brooke Coffman | 15.07 | 2023 |
| 3 | Morgan Goode | 15.50 | 2002 |
| 4 | Chris Teike | 15.59 | 1987 |
| Morgan Sandrock | 15.59 | 2023 |
| 6 | Ally Parramore | 15.75 | 2016 |
| 7 | Debbie Baker | 15.80 | 1985 |
| 8 | Stephanie Mariani | 15.90 | 1998 |
| 9 | Jerica Richter | 16.00 | 2009 |
| 10 | Tammy Frost | 16.10 | 2000 |
| 11 | Casey Macharia | 16.39 | 2024 |
| 12 | Jill Hearin | 16.40 | 1980 |
| 13 | Megan Woods | 16.50 | 2016 |
| Lauren Sandrock | 16.50 | 2021 |
| 15 | Jennifer Wagers | 16.56 | 1994 |
| 16 | Katie Gordon | 16.75 | 2004 |
| 17 | Dawnette Plunkett | 16.91 | 1983 |
| 18 | Emma Beavins | 16.97 | 2015 |
| 19 | Suzanne Leighty | 17.00 | 1984 |
| Michelle Cummings | 17.00 | 1995 |
| 21 | Hali Parker | 17.05 | 2010 |
| 22 | Kerrie Smith | 17.40 | 1991 |
| 23 | Susan Aldrich | 17.60 | 1992 |
| 24 | Carly Sandrock | 17.61 | 2014 |
| 25 | Tina Spicer | 17.63 | 1993 |