| Rank | Team | Time | Year |
| 1 | Cami Kelsay, Rachel Simmons, Emma Beavins, Kloie Doublin | 49.38 | 2018 |
| Aubrey Runyon, Addie Martin, Hanna Stewart, Sami Frazier | 49.38 | 2024 | |
| 3 | Ellie Stringer, Jennifer Briggs, Brooke McClure, Erica Long | 49.82 | 1997 |
| 4 | Jennifer Stone, Christina Thompson, Kristen Chiarotti, Dawn Golden | 50.00 | 1989 |
| 5 | Morgan Hoy, Megan Woods, Kaitlyn Novak, Rachel Simmons | 50.84 | 2017 |
| 6 | Jennifer Stone, Chris Teike, Kristen Chairotti, Shannon Eades | 51.10 | 1988 |
| 7 | Natalie McWhorter, Randi Stock, Kylie Burgess, Erin Bullington | 51.30 | 2003 |
| 8 | Ellie Stringer, Brooke McClure, Jennifer Briggs, Andrea White | 51.60 | 1998 |
| 9 | Debbie Pruitt, Debbie Baker, Jamie Logan, Ann Golden | 51.69 | 1984 |
| 10 | Humes, Olsen, Baker, Charlotte Linville | 51.70 | 1982 |
| Erica Long, Carrie Reese, Brooke McClure, Michelle Cummings | 51.70 | 1996 | |
| 12 | Morgan Sandrock, Casey Macharia, Hailey Stewart, Samantha Frazier | 51.76 | 2023 |
| 13 | Aubrey Runyon, Ava Lenkey, Sabina Garcia, Olivia Nicoloff | 51.92 | 2022 |
| 14 | Katrina Emry, Kelly Clarkston, Jennifer Briggs, Natalie McWhorter | 52.04 | 2000 |
| 15 | Stacy Blotch, Brooke McClure, Brooke Clarkston, Erica Long | 52.10 | 1995 |
| 16 | Sarah Cearing, Angela Shaul, Kylie Burgess, Erin Bullington | 52.14 | 2004 |
| 17 | Jennifer Stone, Chris Teike, Kristen Chairotti, Shannon Eades | 52.15 | 1987 |
| 18 | Jerica Richter, Angela Shaul, Erin Bullington, Kylie Burgess | 52.28 | 2006 |
| 19 | Sarah Cearing, Angela Shaul, Kylie Burgess, Erin Bullington | 52.31 | 2005 |
| 20 | Morgan Hoy, Megan Woods, Katie Novak, Rachel Simmons | 52.38 | 2016 |
| 21 | No recorded names | 52.40 | 1986 |
| 22 | Michelle Baker, Shatto, Logan, Eades | 52.48 | 1985 |
| 23 | Jerica Richter, Rebecca Allee, Destinee Smith, Tori Hamner | 52.53 | 2009 |
| 24 | Morgan Hoy, Carly Sandrock, Emma Beavins, Kloie Doublin | 52.58 | 2015 |
| 25 | Repp, Lucas, Records, Hearin | 52.60 | 1980 |
| Rank | Team | Time | Year |
| 1 | Aaron Scott, James Pfiefer, Jake Moran, Brian Murr | 42.86 | 2012 |
| 2 | Scott, Pfifer, Ballay, Murr | 43.26 | 2011 |
| 3 | Hall, Shephard, Packer, Jordan | 43.50 | 1989 |
| 4 | Ethan Elsner, Elijah Smith, Aidan Jefry, Dylan Beverly | 43.77 | 2023 |
| 5 | Hunter, McCarty, Martin, Shephard | 43.90 | 1992 |
| Giddens, Clark, Warner, B. Murr | 43.90 | 2010 | |
| 7 | Jordan, Packer, Shephard, Hall | 44.01 | 1988 |
| 8 | N. Belser, C. Thompson, J. Weinhorst, S. Whittenbarger | 44:04 | 1998 |
| 9 | Price, Yaden, Epperson, Hunter | 44.22 | 1993 |
| 10 | Jones, McCann, Harris, Murr | 44.23 | 2017 |
| 11 | M. Sever, Richards, Mosburg, Hunter | 44.68 | 1995 |
| 12 | Kocher, Thompson, Wienhorst, Whittenbarger | 44.75 | 1997 |
| 13 | J. Smith, B. Murr, Makransky, Moran | 44.80 | 2009 |
| 14 | M. Nickleson, A. Freese-Gastil, A. Jefry, G. Hueston | 44.85 | 2022 |
| 15 | Gardner, Madison, Nibarger, J. Williams | 45.10 | 2006 |
| 16 | B. Epperson, E. Richards, B. Yaden, M. Cowden | 45.10 | 1994 |
| 17 | J. Cox, M. Chadbourne, A. Shepherd, Z. Dowell | 45.2 | 2014 |
| 18 | A. Loos, C. Mills, K. Hamblin, R. McCann | 45.27 | 2018 |
| 19 | D. Utterback, T. Reed, J. Cox, T. Murr | 45.32 | 2015 |
| 20 | Baumgardner, Hamner, Turner, Wagner | 45.36 | 2002 |
| 21 | Deckard, Whittenbarger, Stevenson, Huddleston | 45.33 | 1999 |
| 22 | Nibarger, Montalvo, Noblitt, Madison | 45.40 | 2005 |
| 23 | Z. Dowell, A. Ballay, A. Shepherd, J. Cox | 45.42 | 2013 |
| 24 | Hamner, Montalvo, Walls, Young | 45.47 | 2003 |
| 25 | Turner, Green, Svenback, White | 45.50 | 1981 |