| Rank | Name | Time | Year |
| 1 | Lauren Klem | :58.92 | 2022 |
| 2 | Sarah Craciunoiu | :59.60 | 1994 |
| 3 | Christina Linville | 1:00.00 | 1981 |
| 4 | Ainsley Botkin | 1:00.09 | 2023 |
| 5 | Kloie Doublin | 1:00.19 | 2017 |
| 6 | Carrie Reese | 1:00.80 | 1997 |
| 7 | Erica Long | 1:00.85 | 1997 |
| 8 | Maggie Auger | 1:01.12 | 2019 |
| 9 | Christy Shook | 1:01.23 | 2000 |
| 10 | Kathleen Lacy | 1:01.27 | 2022 |
| 11 | Hanna Stewart | 1:01.54 | 2024 |
| 12 | Liz Clark | 1:01.76 | 2006 |
| 13 | Alyssa Henderson | 1:01.78 | 2019 |
| 14 | McKinley Purdy | 1:01.97 | 2015 |
| 15 | Ashley Bechman | 1:02.77 | 2004 |
| 16 | Michelle Baker | 1:02.80 | 1984 |
| 17 | Suzanne Duckworth | 1:02.90 | 1998 |
| 18 | Lavonda Hendrickson | 1:03.00 | 1985 |
| 19 | Morgan Pursifull | 1:03.23 | 2010 |
| 20 | Hailey Stewart | 1:03.35 | 2021 |
| 21 | Cami Kelsay | 1:03.6 | 2021 |
| 22 | Almeda Burton | 01:03.8 | 1987 |
| 23 | Christy Walters | 1:03.90 | 1993 |
| 24 | Cindy Baker | 1:04.00 | 1982 |
| 25 | Rachel Cain | 1:04.06 | 2002 |
| Rank | Name | Time | Year |
| 1 | A. Dunn | 49.16 | 2006 |
| 2 | K. Fechtman | 49.50 | 1997 |
| 3 | H. Chadwell | 50.4 | 2014 |
| G. Hart | 50.4* | 1973 |
| R. Dougherty | 50.4* | 1969 |
| T. Walters | 50.4* | 1966 |
| 7 | T. Fromer | 50.53 | 2018 |
| 8 | E. Reece | 50.93 | 1989 |
| 9 | J. Pfifer | 51.12 | 2010 |
| 10 | S. White | 51.55 | 1995 |
| 11 | N. Stevenson | 51.6 | 2021 |
| 12 | D. Purdy | 51.67 | 2018 |
| 13 | D. Deboy | 51.70 | 2000 |
| 14 | K. Houston | 52.10 | 1983 |
| C. Duckworth | 52.10 | 1998 |
| 16 | A. Clark | 52.14 | 2009 |
| 17 | J. Coy | 52.19 | 1998 |
| 18 | I. Adams | 52.30 | 1989 |
| 19 | J. Compton | 52.50 | 2005 |
| M. Dunn | 52.60* | 1957 |
| 21 | B. Betts | 52.70 | 1994 |
| 22 | J. Stevenson | 52.90 | 1998 |
| 23 | G. Hueseon | 52.94 | 2021 |
| 24 | P. Ruble | 53.22 | 2019 |
| 25 | J. Reeves | 53.27 | 1987 |
| | | |
| *=Converted times | |