Rank | Name | Time | Year |
1 | Erica Long | 25.10 | 1997 |
2 | Cami Kelsay | 26.10 | 2018 |
3 | Samantha Frazier | 26.38 | 2024 |
4 | Kloie Doublin | 26.39 | 2018 |
5 | Jennifer Stone | 26.40 | 1988 |
6 | Natalie McWhorter | 26.48 | 2003 |
7 | Anne Golden | 26.73 | 1984 |
8 | Erin Bullington | 26.75 | 2005 |
9 | Lauren Klem | 26.76 | 2024 |
10 | Ellie Stringer | 26.78 | 1997 |
11 | Addie Martin | 27.0 | 2024 |
12 | Olivia Nicoloff | 27.07 | 2022 |
13 | Jennifer Briggs | 27.10 | 1998 |
14 | Dolly VanCleave | 27.20 | 1982 |
| Christina Thompson | 27.20 | 1991 |
16 | Shannon Eades | 27.24 | 1988 |
17 | Ainsley Botkin | 27.29 | 2024 |
18 | Angie Kutruff | 27.30 | 1982 |
19 | Tori Hamner | 27.40 | 2009 |
20 | Brooke McClure | 27.43 | 1997 |
21 | Sara Doyle | 27.50 | 1982 |
| Christina Linville | 27.50 | 1982 |
23 | Chris Teike | 27.52 | 1987 |
24 | Sarah Craciunoiu | 27.54 | 1994 |
25 | Destinee Smith | 27.62 | 2009 |
Rank | Name | Time | Year |
1 | Brian Murr | 21.74 | 2011 |
2 | Richard Jordan | 21.90 | 1989 |
3 | Brent Waymire | 22.1* | 1972 |
| R. Dougherty | 22.1* | 1967 |
5 | T. Murr | 22.40 | 2017 |
| D. Clarkston | 22.4* | 1966 |
7 | B. Hunter | 22.50 | 1993 |
| A. Dunn | 22.50 | 2007 |
9 | K. Fechtman | 22.53 | 1997 |
| M. Findley | 22.70* | 1969 |
11 | S. Packer | 22.79 | 1989 |
12 | B. Murr | 22.90 | 2010 |
13 | J. Moran | 22.95 | 2009 |
14 | R. McCann | 23.05 | 2018 |
15 | G. White | 23.10 | 1981 |
16 | J. Cox | 23.14 | 2015 |
17 | N. Hamner | 23.33 | 2003 |
18 | S. Crenshaw | 23.40 | 1982 |
| R. Montalvo | 23.40 | 2004 |
20 | J. Weinhorst | 23.41 | 1998 |
21 | B. Shepard | 23.50 | 1988 |
| C. Wagner | 23.50 | 2002 |
23 | J. Whittenbarger | 23.52 | 1998 |
24 | D. Deboy | 23.59 | 2000 |
25 | H. Chadwell | 23.60 | 2015 |
| *=Converted times | |