| Rank | Team | Time | Year |
| 1 | Aubrey Runyon, Hanna Stewart, Ainsley Botkin, Lauren Klem | 4:00.42 | 2024 |
| 2 | Aubrey Runyon, Ainsley Botkin, Brooke Coffman, Lauren Klem | 4:03.85 | 2023 |
| 3 | Olivia Nicoloff, Ainsley Botkin, Brooke Coffman, Lauren Klem | 4:04.04 | 2022 |
| 4 | Ally Parramore, Emma Beavins, Kloie Doublin, McKinley Purdy | 4:07.99 | 2016 |
| 5 | Kloie Doublin, Ally Parramore, Maggie Auger, Emma Beavins | 4:09.72 | 2017 |
| 6 | Carrie Reese, Amy Stenger, Kelly Clarkston, Erica Long | 4:09.82 | 1997 |
| 7 | Alyssa Henderson, Lillian Lacy, Macy Doublin, Hailey Stewart | 4:13.19 | 2021 |
| 8 | Jennifer Burton, Christy Walters, Tina Spicer, Sarah Craciunoiu | 4:13.40 | 1994 |
| 9 | Christy Walters, Sarah Craciunoiu, Tina Spicer, Jennifer Burton | 4:13.60 | 1993 |
| 10 | Carrie Reese, Kimere Corthell,Suzanne Duckworth, Sara Moyer | 4:14.59 | 1998 |
| 11 | Erin Bullington, Katie Walls, Liz Clark, Kylie Burgess | 4:14.52 | 2005 |
| 12 | Carrie Reese, Michelle Cummings, Amy Stenger, Erica Long | 4:14.80 | 1996 |
| 13 | Morgan Goode, Jill Heminger, Ashley Gilles, Christi Shook | 4:15.47 | 2000 |
| 14 | Morgan Goode, Jill Heminger, Jenny Jessen, Christi Shook | 4:15.60 | 2001 |
| 15 | Rachel Cain, Morgan Goode, Ashley Gilles, Chrsti Shook | 4:15.90 | 1999 |
| 16 | Erin Bullington, Kylie Burgess, Ashley Bechman, Liz Clark | 4:16.11 | 2006 |
| 17 | Ally Parramore, Carly Sandrock, Khloie Doublin, McKinley Purdy | 4:16.61 | 2015 |
| 18 | Christi Shook, Ashley Adams, Rachel Cain, Morgan Goode | 4:17.86 | 2002 |
| 19 | McKinley Purdy, Alexis Hash, Mia Cano, Brittany Ward | 4:18.41 | 2013 |
| 20 | Maggie Auger, Liv Parramore, Chloe Selking, Emma Beavins | 4:18.56 | 2018 |
| 21 | Alyssa Henderson, Lauren Sandrock, Hailey Doddridge, Maggie Auger | 4:18.73 | 2019 |
| 22 | Lavonda Hendrickson, Debbie Baker, Debbie Pruitt, Cheryl Price | 4:20.50 | 1985 |
| 23 | Courtney Clarkston, Michelle Cummings, Amy Stenger, Carrie Reese | 4:20.70 | 1995 |
| 24 | No recorded names | 4:21.91 | 1986 |
| 25 | Morgan Pursifull, Paige Hendrix, Meagan Hash, Hali Parker | 4:22.07 | 2010 |